IET nanobiotechnol. Carvacrol is a compound of many aromatic plants that are usually used as spices in culinary and for therapy/prevention purposes in folk medicine. doi:10.1016/j.phymed.2013.01.015. doi:10.1136/jmg.2009.066944, Ghorani, V., Alavinezhad, A., Rajabi, O., Mohammadpour, A. H., and Boskabady, M. H. (2021). 44 (2), 177–189. National Center for Biotechnology Information. Este método fue llevado por el método recuperación después de la adición de dos concentraciones de estándar 0,1 y 1,0 ug/mL de carvacrol y 0,7 y 7,0 μg/mL de timol. Adv. MIC 90 values ranged from 0.015 to 0.03% (146-292 μg/mL) for the Gram-positive bacteria and P. mirabilis. Pharmaceuticals 13 (11), 405. doi:10.3390/ph13110405, Sivaranjani, A., Sivagami, G., and Nalini, N. (2016). Carvacrol es un químico que contiene propiedades de calentamiento. 7. carvacrol oregano emulsificado Ordenar por Más relevantes Aceite Esencial De Oregano Natier 30ml 70% Carvacrol 3140 pesos$3.140 Primal Fx Oregano Oil 80% Carvacrol Timol. Nat. Genoprotective Effects of Essential Oil Compounds against Oxidative and Methylated DNA Damage in Human Colon Cancer Cells. (2004). Mol. (2016). Hypothese/ziele: Se secó a temperatura ambiente en sombra, obteniéndose 200 g de hojas secas. Entre las propiedades del orégano se ha visto que puede destruir bacterias o impedir su crecimiento. A principal via metabólica é a esterificação do grupo fenólico com ácido sulfúrico e ácido glucurónico. Carvacrol supplementation significantly improved the weight gain and growth rate of animals with colon cancer induced by 1,2-dimethylhydrazine (DMH), exhibiting a lower incidence of tumors and pre-neoplastic lesions, along with a reduction in oxidative stress damage (higher levels of GSH, GPx, GR, SOD and CAT), suggesting that carvacrol presents chemopreventive effects (Sivaranjani et al., 2016). Se obtuvo el cromatograma de la figura 4, donde la concentración de carvacrol fue de 0,003 mg/L y la de timol fue de 0,189 mg/L. Nat. Toxicol. In Vitro effects of Two Major Phenolic Compounds from the Family Lamiaceae Plants on the Human Gastric Carcinoma Cells. CDK4/6 Inhibition in Breast Cancer: Current Practice and Future Directions. (2019). Bioorg. A time-kill kinetics assay was performed to confirm the bactericidal and fungicidal activity of the oils and their phenolic constituents. However, a phase I randomized clinical trial conducted in healthy subjects treated with carvacrol (1 or 2 mg/kg daily, p.o., during one month) revealed that this monoterpene did not lead to clinically significant changes, and did not cause any adverse effects, showing clinical safety and tolerability for this agent (Ghorani et al., 2021). Biophysical Res. Rev. Oncogene 18 (10), 1781–1787. Clinical Significance of Plasma MMP‐2 and MMP‐9 Levels as Biomarkers for Tumor Expression in Breast Cancer Patients in Egypt. Prod. Carvacrol is a compound found in various herbs including oregano and oregano essential oil. In particular, JNK, ERK and p38 are the main proteins of MAPK pathways when approaching cancer. In mice, the lethal dose was 110–233.3 mg/kg, after inducing ataxia and drowsiness (Suntres et al., 2015). (2014). Significance and impact of study: In the wake of rising antimicrobial resistance, we envisage that carvacrol and thymol could be used to prevent biofilm formation by UPEC and to reduce its virulence. Este compuesto tiene un olor aromático agradable, y podemos extraerlo de varias plantas como un sólido cristalino blanco. in male Wistar rats provided promising protective activity against colon cancer by significantly reducing elevated serum levels of colon-related tumor markers, carbohydrate antigen 19-9 (CA 19-9) and carcinoembryonic antigen (CEA), as well as the apoptotic marker, caspase-3 compared to the colon cancer group. 14, 2535–2547. As for the lung metastasis model, HCT116 cells were injected into the tail vein of each mouse and then received treatment with thymol (75 or 150 mg/kg every other day). (2005). doi:10.1371/journal.pmed.1003583, Pakdemirli, A., Karaca, C., Sever, T., Daşkin, E., Leblebici, A., Yiğitbaşi, T., et al. Pharmacol. Int. ¿Qué es el carvacrol? (2018). [13], Um estudo conduzido por Supriya Bavadekar reporta que o carvacrol estimula a apoptose de células do cancro da próstata.[14]. In contrast, post-treatment with carvacrol demonstrated the presence of small persistent nodules, loss of cellular architecture and a lower tendency to spread through the intrahepatic veins. Cel Biochem. Res. Instituto de Salud Pública. Se extrajo durante 120 minutos. doi:10.1049/iet-nbt.2014.0040, Mehdi, S., Ahmad, A., Irshad, M., Manzoor, N., and Rizvi, M. (2011). 38 (4), 263–355. E.Katz, R.Eksteen, P. Schoenmakers, N. Miller. Food Chem. (2015). It was possible to verify that, in general, carvacrol (336.7 ± 35.0, n = 21) is more potent than thymol (527.1 ± 146.6, n = 11), with difference between means of 103.8 (±106.9). It is important to note that carvacrol prevented metastasis in vivo, demonstrated by tumors less likely to spread through intrahepatic veins (Jayakumar et al., 2012) and preventing local spread of cancer cells by suppressing the expression of MMP-2 and MMP-9 proteins (Subramaniyan et al., 2014), and thymol decreased the number of lung metastatic lesions in vivo, suppressing the Wnt/β-catenin signaling pathway (Zeng et al., 2020). Thus, inhibition of the PI3K/Akt/mTOR signaling pathway may be a possible mechanism involved behind the effects of thymol on HL-60 cells (Pathania et al., 2013). doi:10.1023/b:cyto.0000039917.60348.45. El método propuesto consiste de un sistema HPLC con detector de Fluorescencia, fase móvil isocrática de acetonitrilo y agua (ACN):H2O (50:50), columna Purospher® STAR rp-18e (4,6 x 150 mm, 5 ìm), flujo de 1 mL/min, volumen de inyección de 20 ìL y un tiempo de corrida de 15 minutos. RESUMEN. J. Pharmacol. Hypothesis/objectives: In this review, it was noted that three in vitro studies (4.4%) reported that carvacrol decreased CDK4 protein expression in human tongue squamous cell carcinoma (Tca-8113 cells) (Dai et al., 2016) and in breast cancer (MCF-7 cells) (Mari et al., 2020; Li et al., 2021) and only one (1.4%) reported a decrease in CDK6 in MCF-7 cells (Mari et al., 2020). J. The Cytotoxic Effects of Thymol as the Major Component of Trachyspermum Ammi on Breast Cancer (MCF-7) Cells. It was hypothesized that the main phenolic components would have greater antimicrobial activity compared to the relative essential oil. In tongue cancer (Tca-8113, SCC-25 cell lines), Dai et al. The increased expression of VEGF during cancer, along with other pro-angiogenic factors, is responsible for new vascularization, representing a strategic point in the treatment (Collins and Hurwitz, 2005; Riaz et al., 2015; Siveen et al., 2017). doi:10.21037/jtd.2019.08.92, Zarubin, T., and Han, J. An official website of the United States government. Mod. 2 (5), 2367–2396. Infect. En particular, el carvacrol y el timol son dos compuestos del orégano que se han asociado con propiedades antivirales. Further studies are needed to clarify this issue. Bras. J. Respir. The pre- and post-treatment with carvacrol in animals with liver cancer induced by diethylnitrosamine (DEN) revealed a decrease in the number of nodules, a final body weight increase and a reduction in liver weight. They were mostly obtained commercially, provided by Sigma Aldrich (n = 52), Fluka (n = 5), Aldrich Chemical (n = 2), Western Chemical (n = 1), Agolin SA (n = 1), Alfa Aesar (n = 1). J. Carvacrol also induced cytotoxicity and apoptosis (via caspase-3 and reactive oxygen species—ROS) of human oral squamous cell carcinoma (OC2 cell line) in a concentration-dependent manner (Liang et al., 2013). We extracted the following data from the included articles: author, year, country, data about the monoterpene (source, obtention method), concentration and/or dose, type of animal or cell line, results (cytotoxicity, cell proliferation, apoptosis, cell cycle, histology), proposed mechanisms involved in the antitumor effect and conclusion. CASPASE-8 Gene Is Inactivated by Somatic Mutations in Gastric Carcinomas. The generation of ROS inside the cell contributes to the antitumor process in order to induce DNA damage and slow the progression of the cell cycle, preventing cells with DNA damage (cancer cells) from continuing with cell division (Allawzi et al., 2019; Srinivas et al., 2019). Regarding thymol, treatment at concentrations of 100 and 200 µM induced a significant reduction in cell viability and inhibited the migration of glioma cells (C6 cell line) through phosphorylation of PKCα and ERK1/2, that resulted in decreased expression of MMP-9 and MMP-2 (Lee et al., 2016). óleo essencial de orégano (OEO) são o carvacrol e o timol com comprovado efeito inibitório no controle in vitro da multiplicação de Salmonella (RHAYOUR et al., 2003; NAZER et al., 2005; SILVA et al., 2005). El carvacrol es un compuesto químico que se encuentra en los aceites esenciales de la mejorana, el orégano, el tomillo, la ajedrea y otras plantas. Methodol. (2000). (2018). Of these, three were excluded after reading the full text (two for presenting the same results and one that tested the anti-cancer effect on bacteria) and four for not having access to the full text, resulting in 70 articles. Exp. 35 (2011), 735–742. Pour P. aeruginosa et M. pachydermatis, les valeurs de MIC90 allaient de 0.09 à 0.25% (800-2,292 μg/mL). doi:10.2174/156652411800615162, Belyi, V. A., Ak, P., Markert, E., Wang, H., Hu, W., Puzio-Kuter, A., et al. 5815 pesos $ 5.815. Drugs 28 (11), 989–1001. Para el dolor y la inflamación. A narrative summary of the results is presented below, divided into in vitro and in vivo studies. 10 (1), 61–64. It is of note that several studies suggested that signaling pathways (PI3K/AKT/mTOR and MAPKs) may be the main mechanism of action of these monoterpenoids, but more advanced studies are needed to elucidate this issue. During cancer, overexpression of anti-apoptotic genes and under expression of pro-apoptotic genes can lead to the failure of the programmed cell death mechanism (Olsson and Zhivotovsky, 2011; Zhang et al., 2000). The History of Medicine. Clin. J. Med. Effect of Neoplasms on the Content and Activity of Alkaline Phosphatase and Gamma-Glutamyl Transpeptidase in Uninvolved Host Tissues. Los límites de detección determinado LOD y límite de cuantificación LOQ fue de 0,0007 - 0,002 mg/L para el timol y 0,002 - 0,005 mg/L para el carvacrol, con una recuperación de 98,68 % y 90,95 %, respectivamente. Its inhibition was identified by 2.8% (n = 2) of in vitro studies using thymol in human promyelocytic leukemia cell lines (Pathania et al., 2013) and bladder cancer cells (Li et al., 2017), and by 7.2% (n = 5) of in vitro studies that used carvacrol in colon cancer (Fan et al., 2015), glioblastoma (Chen et al., 2015), prostate cancer (Luo et al., 2016), choriocarcinoma (Lim et al., 2019) and breast cancer cells (Mari et al., 2020). Proibida a cópia ou reprodução sem autorização prévia. Prepara un aceite de orégano o diluye el aceite esencial de romero en aceite base (de almendras dulces . Se colectó hojas de Origanum vulgare provenientes de zonas de cultivo en Tacna, Perú. Treatment with carvacrol increased p53 expression in breast cancer (Al-Fatlawi and Ahmad, 2014), cervical and liver cancer cells (Abbas and Al-Fatlawi, 2018). doi:10.1016/j.semcancer.2017.04.015, Olsson, M., and Zhivotovsky, B. In vitro efficacy of oregano oil, thyme oil and their main phenolic constituents against bacterial and fungal isolates associated with canine otitis externa were investigated. Antimicrobial Activity of Basil, Oregano, and Thyme Essential Oils. Natural Compounds as Modulators of Cell Cycle Arrest: Application for Anticancer Chemotherapies. This systematic review was registered in the PROSPERO database (CRD42020176736) and the protocol is available at https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=176736. 69 (5), 1057–1065. Medicinal Plants for Women's Healthcare in Southeast Asia: A Meta-Analysis of Their Traditional Use, Chemical Constituents, and Pharmacology. L. P. Roldán. 62, 246–254. doi:10.1002/ptr.6103, Sheorain, J., Mehra, M., Thakur, R., Grewal, S., and Kumari, S. (2019). Neoplasma 61 (6), 690–699. There was also inhibition of HCT116, LoVo and HT-29 cells proliferation (Fan et al., 2015; Pakdemirli et al., 2020). Es wirkt sich positiv auf den gesamten Prozess der Wundheilung, aus. CONCLUSÕES E SIGNIFICÂNCIA CLÍNICA: O óleo de orégano, óleo de tomilho, carvacrol e timol demonstraram boa atividade bactericida e fungicida in vitro contra os 100 isolados de cães com otite externa, incluindo isolados altamente multirresistentes. Horvathova, E., Turcaniova, V., and Slamenova, D. (2007). 14, 43. doi:10.1186/1471-2288-14-43, Horng, C.-T., Chou, C., Sun, T., Liang, W., Kuo, C., Wang, J., et al. doi:10.1016/j.fct.2019.01.003. 59, 125–132. 60 (1), 32–40. Curr. In glioblastoma (cell line U87), carvacrol induced apoptosis by increasing the levels of caspase-3 cleavage, moreover, its antitumor mechanism of action seems to be related to the inhibition of PI3K/Akt signaling pathways, activation of mitogen/protein kinase by extracellular signals (via MAPK/ERK) and decreased levels of MMP-2 protein (Chen et al., 2015). Thymol also caused a decrease in cell viability of prostate cancer (PC-3 cell line), and provoked cytotoxic effects (Pathania et al., 2013; Yeh et al., 2017; De La Chapa et al., 2018). Toxicol. doi:10.2174/1871520617666170327121228, Chen, W.-L., Barszczyk, A., Turlova, E., Deurloo, M., Liu, B., Yang, B. doi:10.1007/s11094-019-01961-w, Shalini, S., Dorstyn, L., Dawar, S., and Kumar, S. (2015). 55 (3), 206–211. 1. 360 (1–2), 51–60. (1982). 2011; 43 (1): 237-245. Elimina Cándida. doi:10.1111/j.1349-7006.2010.01524.x, Ingham, M., and Schwartz, G. K. (2017). National Institutes of Health. (2021) that at a concentration of 300 µM of carvacrol the KG1 cell lines were more sensitive compared to the HL60 cell line, and at 400 µM the K-562 cell line showed resistance after 48 h of treatment. Commun. 125, 198–209. The expression of proapoptotic proteins, such as Bax (Li et al., 2017; Basu and Haldar, 1998), is an important mechanism of tumor regression (Backus et al., 2002). Vet Dermatol. Biophysical Res. 670 (1), 85–91. (2018). (2010). Determinación de timol y carvacrol en una matriz orgánica de alimento . Cancer 3 (10), 721–732. doi:10.1021/jf204525b, Semenza, G. L. (2003). Thymol and Carvacrol also have antibacterial properties as pure substances. doi:10.1080/10408398.2011.653458, Swanton, E., Savory, P., Cosulich, S., Clarke, P., and Woodman, P. (1999). Res. (2004). (2018). It is estimated that one in five people worldwide faces the diagnosis of some malignant neoplasm during their lifetime, and the number of people with cancer is forecast to double by the year 2040 (World Health Organization, 2020). doi:10.1016/j.cotox.2018.08.001, Alrehaili, A. (2019) showed that tumor growth in mice with DEN-induced hepatocarcinoma and treated with carvacrol was limited, revealing tumor cell reduction, rare mitotic figures, normal arrangement of cells, few microvessels, a central necrotic area on tumor tissue and a reduction of intrastromal and peritumor lymphocytes. IC50 values determined 24 h after the incubation of the studied cells with carvacrol or thymol were compiled, submitted to the standardization of the unit (μM) (Supplementary Table S2) and were expressed as mean ± standard error of the mean (SEM). 15 (1), 11–18. Res. Varios estudios han probado la capacidad del aceite de orégano para aliviar el dolor de golpes, esguinces, tendinitis y artritis. 9 (12), 767–776. Crit. Caspase-8 Deficiency Facilitates Cellular Transformation In Vitro. Carvacrol Exhibits Anti-oxidant and Anti-inflammatory Effects against 1, 2-dimethyl Hydrazine Plus Dextran Sodium Sulfate Induced Inflammation Associated Carcinogenicity in the colon of Fischer 344 Rats. 10. (2015) reported that high caspase-3 expression caused apoptosis of tumor cells, and is significantly associated with better prognosis in patients with non-small cell lung cancer (Yoo et al., 2004) and hepatocellular carcinomas (Huang et al., 2010). For P. aeruginosa and M. pachydermatis, MIC 90 values ranged from 0.09 to 0.25% (800-2,292 μg/mL). Mol. Comparative Study of the Antitumor Effect of Natural Monoterpenes: Relationship to Cell Cycle Analysis. Fiandalo, M. V., and Kyprianou, N. (2012). Aplicación del método a muestras de orégano. Oral Pathol. Contexto: A Comparative Evaluation of Antioxidant and Anticancer Activity of Essential Oil from Origanum Onites (Lamiaceae) and its Two Major Phenolic Components. Natural Products: an Evolving Role in Future Drug Discovery. 4. Rev. Perspect. Background: It is estimated that one in five people worldwide faces a diagnosis of a malignant neoplasm during their lifetime. O conteúdo publicado aqui é destinado única e exclusivamente para fins educacionais, de pesquisa e informação e não deve ser utilizado como substituto ao diagnóstico médico ou tratamento sem consulta prévia ao profissional de saúde. Before Cancer 69 (7), 1075–1087. Nicolás de Araníbar 696 Santa Beatriz - Lima 01, Revista de la Sociedad Química del Perú. Turk J. Med. However, the toxicity and side effects of many treatments can worsen the quality of life of these individuals (Weingart et al., 2018; Hassen et al., 2019; Lu et al., 2019). Thymol also showed concentration-dependent cytotoxic effects and reduced the proliferation of Caco-2 cells (Horváthová et al., 2006). (1990). daenensis Celak. En uso interno Antibacteriano. Alpha-Fetoprotein and Hepatocellular Carcinoma Immunity. Detection limits (DL) and quantification limits (QL) were 0.0007 to 0.002 mg/L for, thymol and 0.002 to 0.005 mg/L for carvacrol with a recovery of 98.68% and 90.95% respectively. Carvacrol and its isomer, thymol, are natural compounds that act against several diseases, including cancer. Remarkably, ten studies did not report the source of the tested compounds. Saputra S, Jordan D, Worthing KA et al. Phytomedicine 20 (7), 615–621. Antimicrobial susceptibility testing was performed using broth microdilution with spot-plating technique to determine minimum inhibitory and bactericidal/fungicidal concentrations (MICs, MBCs and MFCs). Sci. In addition, high levels of GGT influence proliferation and apoptosis and contribute to tumor progression (Koss and Greengard, 1982; Pompella et al., 2006; Zhang et al., 2006) serving as a biomarker in various types of cancer (Paolicchi et al., 1996; Whitfield, 2001; Pompella et al., 2006). doi:10.1111/jop.12735, de Sá Junior, P. L., Câmara, D. A. D., Porcacchia, A. S., Fonseca, P. M. M., Jorge, S. D., Araldi, R. P., et al. The uncontrolled proliferation of cancer cells occurs due to their ability to prevent programmed cell death (apoptosis) (Olsson and Zhivotovsky, 2011; Dabrowska et al., 2016; Kim and Kim, 2018), that is responsible for eliminating aberrant proliferating cells or those with DNA damage/mutations (Dabrowska et al., 2016) (Figure 5). Conception and design: AG, DT, and LS. Med. O óleo essencial do orégano possui inúmeras propriedades medicinais, sendo composto basicamente de carvacrol e timol. 12. One hundred bacterial and fungal isolates, including meticillin-susceptible Staphylococcus pseudintermedius (n = 10), meticillin-resistant S. pseudintermedius (n = 10), β-haemolytic Streptococcus spp. Elevated Alpha-Fetoprotein. A blockade of TRPM7 channels, reduced expression of MMP-2 and F-actin, was also observed, together with the inhibition of PI3K/Akt and MAPK (Mitogen-activated protein kinases) signaling pathways was also observed (Luo et al., 2016). Rev. Carvacrol Induced Program Cell Death and Cell Cycle Arrest in Androgen-independent Human Prostate Cancer Cells via Inhibition of Notch Signaling. box-shadow: none; Liolios, O. Gortzi, S. Lalas, J. Tsaknis, I. Chinou; Liposomal incorporation of carvacrol and thymol isolated from the essential oil of Origanum dictamnus L. and in vitro antimicrobial activity. mirabilisの0.015〜0.03%(146〜292μg/ mL)の範囲にわたった。 緑膿菌およびM. En la figura 3 la curva de calibración del carvacrol presenta un valor de r2 de 0,999811 para concentraciones de 0,03 hasta 0,96 mg/L. (2012). Anticancer Drugs 26 (8), 813–823. El carvacrol y el timol son compuestos orgánicos cíclicos que contienen un grupo metilo, un grupo hidroxilo y un grupo isopropilo. doi:10.1016/s0020-7292(96)02770-1, Abed, R. (2011). Immune Netw. Cytotoxic, Cytogenetics and Immunomodulatory Effects of Thymol from Thymus Vulgaris on Cancer and Normal Cell Lines In Vitro and In Vivo. doi:10.1002/jbt.22275, Fundyler, O., Khanna, M., and Smoller, B. R. (2004). These effects occurred mainly through the inhibition of tyrosine kinase receptor (AXL) expression and an increase in malondialdehyde (MDA) and 8-hydroxy-2′-deoxyguanosine levels (8-OHdG) (Ozkan and Erdogan, 2012; Jung et al., 2018). doi:10.1007/s10616-013-9547-5, Ayude, D., Fernández-Rodríguez, J., Rodríguez-Berrocal, F. J., Martínez-Zorzano, V. S., De Carlos, A., Gil, E., et al. Se determinó que los estándares de carvacrol y timol se encuentran bien definidos con tiempos de retención de 9,5 y 10,6 minutos, como se observa en el cromatograma siguiente (figura 1). O óleo de orégano também auxilia no tratamento de gripes e resfriados, febre leve, menstruação dolorosa, além de ser útil no tratamento de uma série de infecções causadas por bactérias. PLoS ONE 10 (8), e0135078. Value of the Serum Alpha-L-Fucosidase Activity in the Diagnosis of Colorectal Cancer. Es un fenol monoterpenoide. ROS and the DNA Damage Response in Cancer. J. Pathol. 5. The following inclusion criteria were applied: Administration of carvacrol or pure thymol vs. placebo; in vitro studies of cancer cell lines, in vivo study of animals with cancer; cytotoxic effect, antitumor effect, inhibition of proliferation and apoptosis and experimental studies (in vitro and in vivo). (2017). Moreover, genotoxic effects have been shown to be exhibited by carvacrol (Günes-Bayir et al., 2018) and thymol (Günes-Bayir et al., 2018), and it was also shown that a high concentration of carvacrol (460 µM) exhibited mutagenic and genotoxic effects causing DNA damage (Llana-Ruiz-Cabello et al., 2015). Rodrigues et al. Treat. 105, 652–661. Es un líquido incoloro, levemente soluble en agua y soluble en etanol y éter. Neville, B. Hanbook of HPLC, Chromatographics science series, V 78: 66-67. Carvacrol Inhibits Proliferation and Induces Apoptosis in Human colon Cancer Cells. doi:10.1517/14728222.2014.961425, Kim, C., and Kim, B. Este proyecto se desarrolló en conjunto entre Molinos Cusco SA y el Centro de Investigación en Química Toxicología y Biotecnología Ambiental (CIQTOBIA) del Departamento Académico de Química y el apoyo del Instituto de Investigación en Bioquímica y Biología Molecular de la UNALM. 2020 Apr;31(2):81-85. doi: 10.1111/vde.12807. (2017). Med. When using mouse myoblast cells (CO25 cell line) transformed with human N-RAS oncogene, Zeytinoglu et al. In addition, the induction of apoptosis in both cell lines occurred in a concentration-dependent manner (Elbe et al., 2020). Bellow this altitude, the concentration of essential oils (thymol and carvacrol) decreases. 8 (2), 105–116. Infeccion Candida 48600 pesos$48.600 Envío gratis Aceite Esencial De Oregano Natier 30ml 70% Carvacrol 3099 pesos$3.099 Aceite De Oregano Organico Carvacrol Timol 30ml Vegano Eeuu 背景: 多剤耐性病原体は、抗菌薬治療における世界的な主要課題であり、犬外耳炎をしばしば複雑化する。 仮説/目的: 本研究の目的は、犬の外耳炎に関連する細菌および真菌分離株に対するオレガノ油、タイム油、およびそれらの主要フェノール成分のin vitroにおける有効性を調査することであった。主要なフェノール成分は、関連精油と比較してより高い抗菌活性を持つと仮定した。 材料と方法: 最小発育阻止濃度および殺菌/殺真菌濃度(MIC、MBC、MFC)決定に対し、spot-plating技術による微量液体希釈法を用いて薬剤感受性試験を実施した。オイルおよびそのフェノール成分の殺菌および殺真菌活性の証明に、time-kill kinetics assayを実施した。外耳炎を有する犬から採材したメチシリン感受性Staphylococcus pseudintermedius(n = 10)、メチシリン耐性S. Phospho-ERK Is a Biomarker of Response to a Synthetic Lethal Drug Combination of Sorafenib and MEK Inhibition in Liver Cancer. In addition, treatment with this monoterpene promoted an accumulation of cells in the sub-G1 phase, indicating that changes in intracellular calcium and ROS generation are related to the antiproliferative effects observed. [11] A aplicação de carvacrol na língua humana, assim como a activação de TRPV3, produz uma sensação de calor. Serum Alpha-L-Fucosidase. (2017). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. Pharmacology 88 (3–4), 201–212. El orégano (Origanum vulgare L.) es una planta aromática originaria del mediterráneo; tradicionalmente es cultivada en la zona sur del Perú. 1889 pesos $ 1.889. Beyond the Semi-synthetic Artemisinin: Metabolic Engineering of Plant-Derived Anti-cancer Drugs. Chemopreventive Effect of Carvacrol on 1,2-dimethylhydrazine Induced Experimental colon Carcinogenesis. Oncol. Toxicol. Mol. doi:10.1038/bjc.2013.812, Prasad, S., Gupta, S. C., and Tyagi, A. K. (2017). Life Sci. Carvacrol and Human Health: A Comprehensive Review. 45, 129–138. Epub 2021 Oct 21. Science 290 (5493), 989–992. Results: A total of 1,170 records were identified, with 77 meeting the established criteria. [6] Daña la membrana celular e inhibe la proliferación . Los límites de detección (LODs) y cuantificación (LOQs) fueron calculados usando las expresiones 3,3ó/s y 10ó/s, respectivamente; en donde ó es la desviación estándar interceptada y s es la pendiente de la curva de calibración. Targeting Vascular Endothelial Growth Factor and Angiogenesis for the Treatment of Colorectal Cancer. Am Fam Physician 2006; 74: 1,510-1,516. doi:10.1007/s11010-018-3329-5, Günes-Bayir, A., Kocyigit, A., Guler, E. M., and Dadak, A. Hintergrund: box-shadow: 0 0 0 2px #fff, 0 0 0 3px #2968C8, 0 0 0 5px rgba(65, 137, 230, 0.3); El carvacrol, junto con el timol, otro componente químico principal en el aceite de Orégano, posee propiedades de limpieza y antioxidantes. Sim JXF, Khazandi M, Pi H, Venter H, Trott DJ, Deo P. J Appl Microbiol. ACEITE DE ORÉGANO 150 MG: Nuestro Aceite de Orégano contiene Carvacrol, Timol y otros Terpenos, antisépticos y antioxidantes naturales, que promueven la Salud Digestiva y Respiratoria. (2013). doi:10.4103/0973-1482.154925, Siveen, K. S., Prabhu, K., Krishnankutty, R., Kuttikrishnan, S., Tsakou, M., Alali, F. Q., et al. Cell. Thymol also promoted cytotoxicity and apoptosis of KLN 205 cells with an IC50 of 421 and 229.68 μM in 48 and 72 h, respectively (Elbe et al., 2020). (2018), Günes-Bayir et al. Its inhibition induces a pronounced anticancer activity (Li et al., 2016). RESULTADOS: el aceite de orégano, el aceite de tomillo, el carvacrol y el timol exhibieron actividad antibacteriana contra todos los aislamientos bacterianos y fúngicos probados. Mercado Libre Argentina - Donde comprar y vender de todo. Hence, in a rat model of hepatocellular carcinoma induced by diethylnitrosamine (DEN), carvacrol treatment promoted DNA fragmentation indicating its potential as an apoptotic agent. doi:10.1016/j.febslet.2006.08.025, Kroemer, G., Dallaporta, B., and Resche-Rigon, M. (1998). La planta del orégano posee además flavonoides, taninos, ácidos fenólicos, aceites esenciales (carvacrol, timol y origaneno) y ácido rosmarínico que le aportan cualidades antioxidantes y . Toxicol. 1), 177–183. (2011). Palabras clave: Aceite esencial, Timol, Carvacrol, Orégano, Origanum, HS-SPME-GC-MS. Recibido: 17/08/2020 120 (5), 8169–8176. Please enable it to take advantage of the complete set of features! PLoS Med 2016; 13: e1002184. doi:10.2174/1389202917666160808125645, Baj, T., Biernasiuk, A., Wróbel, R., and Malm, A. (2019). Cytotoxic Activity of Origanum Vulgare L. On Hepatocellular Carcinoma Cell Line HepG2 and Evaluation of its Biological Activity. En cuanto a las propiedades físicas de este compuesto, tiene el distintivo olor acre y cálido del orégano. Tesis de Magíster en Producción Animal, línea de profundización en nutrición de monogástricos. 35 (25), 2949–2959. var s = doc.createElement('script'); Rev. Made with non-GMO ingredients. Neoplasma 54 (6), 478–483. Caspases are central apoptosis regulators and executors, and thus attractive targets for the development of therapeutic strategies for cancer treatment (Ghavami et al., 2009; Hensley et al., 2013; Fiandalo and Kyprianou, 2012; Shalini et al., 2015). Aparte de eso, también puede disolverse en soluciones alcalinas fuertes debido a su capacidad para desprotonar. It was also observed that the administration of carvacrol provoked cytotoxic and apoptotic effects on HeLa and SiHa cervical cell lines (Mehdi et al., 2011). Carvacrol; Dağ kekiğinin uçucu bileşenleri timol ve carvacrol olarak ayrılıp aktif oksijenle timolun etkinliği doyuma ulaştırılıp Carvacrol'un (C10 H14 O) frekans ve emilimini yükselterek, aktifleyip biyoaktif bir solüsyon haline getiriyoruz. También contiene compuestos como el carvacrol que han demostrado tener propiedades antiinflamatorias. Results: Oregano oil, thyme oil, carvacrol and thymol exhibited antibacterial activity against all bacterial and fungal isolates tested. Chem. PMC Potential Preventive Effect of Carvacrol against Diethylnitrosamine-Induced Hepatocellular Carcinoma in Rats. El timol pertenece al grupo de los terpenos. 65 (3), 815–821. 395 (1), 65–76. (2019). Se adapta muy bien a los valles interandinos sobre los 2600 m.s.n.m. A PICOS strategy (patient or pathology, intervention, control, and other outcomes and type of study) was used based on: P: Animals with cancer or tumor cells; I: Treatment with carvacrol or thymol; C: No treatment, healthy cells or placebo (vehicle); O: Cytotoxic and antitumor effects, induction of apoptosis and inhibition of proliferation; S: Pre-clinical studies in vitro and in vivo. Li et al. Diese essentiellen Öle und ihre hauptsächlichen phenolischen Bestandteile haben das Potential in vivo für die Therapie einer Otitis externa des Hundes weiter untersucht zu werden. 13 (13), 68–73. 55 (3), 273–279. Cancer Sci. The Roles of ROS in Cancer Heterogeneity and Therapy. Similarly, most of the SYRCLE tool domains for animal studies (random sequence generation; baseline characteristics; allocation concealment; blinding of caregivers and researchers; random evaluation of results; blinding of the results evaluator) were classified as uncertain due to the lack of information in the articles. Ethnopharmacology of Medicinal Plants—Asia and the Pacific | Christophe Wiart | Springer. CDK inhibitor drugs are being used in some cancer types (Mari et al., 2020), such as acute myeloid leukemia (Lee and Zeidner, 2019) and breast cancer (Pernas et al., 2018). In addition, 9 studies were identified after a manual search of the references and two studies obtained from other sources, but only seven studies were added (two studies were excluded for having a mixture between carvacrol and thymol, one for being associated with other substances, and one that studied the extract of a plant rich in thymol), finally resulting in 77 included studies (Figure 1). Together with VEGF, other enzymes collaborate for neoplastic invasion, such as mitochondrial membrane potential. Costa LV, Moreira JMAR, Menezes IG, Dutra V, de Almeida ADBPF. longevity 2017, 2467940. doi:10.1155/2017/2467940, Deb, D. D., Parimala, G., Saravana Devi, S., and Chakraborty, T. (2011). 19 (11), 902–912. doi:10.1371/journal.pone.0222629, Heidarian, E., and Keloushadi, M. (2019). A Useful Marker in the Diagnosis of Hepatocellular Carcinoma. (2014). ltellez@lamolina.edu.pe 2 Molinos Cusco SA, Kilometro 76, Cusipata, Quispicanchi, Cusco 3 Instituto de Investigación en Bioquímica y Biología Molecular de la UNALM Lima –Perú. Ces huiles essentielles et leurs constituants phénoliques principaux ont le potentiel d’être étudiés in vivo pour le traitement des otites externes canines. Thymol Induces Mitochondrial Pathway-Mediated Apoptosis via ROS Generation, Macromolecular Damage and SOD Diminution in A549 Cells. In addition, carvacrol showed a reduction in serum levels of alpha-fetoprotein (AFP), alpha l-fucosidase (AFU), vascular endothelial growth factor (VEGF) and decreased expression of the gamma glutamyl transferase (GGT) gene (Ahmed et al., 2013). Food Sci. A diferencia del carvacrol, el timol es ligeramente soluble en agua a valores de pH neutros. Key words: Oregano, Thymol, Carvacrol, essential oils, HPLC. HIPÓTESIS/OBJETIVOS: Se investigó la eficacia in vitro del aceite de orégano, el aceite de tomillo y sus principales componentes fenólicos contra los aislados bacterianos y fúngicos asociados con la otitis externa canina. doi:10.1111/jcmm.12760, Zeng, Q., Che, Y., Zhang, Y., Chen, M., Guo, Q., and Zhang, W. (2020). Genet. The development of this method is essential to provide tools for decision making to identify regions where one or more ecotypes can potentially grow and produce high quality of essential oils contents of thymol and carvacrol. doi:10.1111/jcmm.13661, Li, H., Hu, J., Wu, S., Wang, L., Cao, X., Zhang, X., et al. doi:10.1159/000331864, Chauhan, A. K., Bahuguna, A., Paul, S., and Kang, S. C. (2018). Ther. Vascular Endothelial Growth Factor (VEGF) Signaling in Tumour Vascularization: Potential and Challenges. Anti-proliferative and Pro-apoptotic Activity of Carvacrol on Human Cancer Cells. The https:// ensures that you are connecting to the (2017) reported HepG2 cell death, decreased cell viability and a selective action of thymol against these tumor cells. Two independent reviewers (L.A.S. In addition, the level of phosphorylated ERK decreased in hepatocarcinoma, colon cancer, choriocarcinoma and prostate cancer cells, after treatment with carvacrol, and p-ERK1/2 levels decreased after thymol treatment in glioma cells (Yin et al., 2012; Fan et al., 2015; Heidarian and Keloushadi, 2019; Lim et al., 2019). Would you like email updates of new search results? O carvacrol pode ser sintetizado pela fusão de cimol-ácido sulfónico com potassa cáustica; pela acção do ácido nitroso sobre 1-metil-2-amino-4-propil benzeno; pelo aquecimento prolongado de cinco partes de cânfora com uma parte de iodo; ou aquecendo carvol com ácido fosfórico glacial ou ainda por meio de desidrogenação de carvona com um catalisador Pd/C. Para el caso de la selectividad se utilizó la resolución de picos cromatográficos 11. J. Agric. Biochem. Sorgo (Sorghum bicolor L. Moench) tratados con timol y carvacrol extraídos de orégano (Lippia graveolens Kunth) mediante métodos químicos, con el fin de obtener fitorreguladores naturales e implementar esta alternativa para minimizar efectos negativos ocasionados por la utilización de compuestos químicos perjudiciales para el . (2018) also reported decreased viability of HeLa cells and induction of apoptosis by PARP cleavage, suggesting that the anticancer effect of thymol is caused by mitochondrial dysfunction and subsequent apoptosis. Farmacogn. Se determinó con la muestras de menor concentración. Biochem. J. Integr. A significant reduction in cancer growth, a greater number of necrotic lesions and a lower level of Ki-67 expression were observed, which reflects cell proliferation. A. Ghasemi Pirbalouti1, M. Rahimmalek, F. Malekpoor, A. Karimi, Variation in antibacterial activity, thymol and carvacrol contents of wild populations of Thymus daenensis subsp. More recently, Rojas-Armas et al. Tem odor característico do orégão (Origanum vulgare). (2021). Anti-Cancer Natural Products and Their Bioactive Compounds Inducing ER Stress-Mediated Apoptosis: A Review. In human osteosarcoma cells (MG63 cell line), thymol reduced cell viability, induced cytotoxic effects and apoptosis, which occurred in a concentration-dependent manner. doi:10.1016/j.cell.2006.01.016, Yeh, J.-H., Chou, C-T., Chen, I-S., Lu, T., Lin, K-L., Yu, C-C., et al. De La Chapa et al. Human glioblastoma cells (DBTRG-05MG) showed reduced viability in a concentration-dependent manner when treated with carvacrol (200–600 μM), induced apoptosis and necrosis by ROS production and caspase-3 activity (Liang and Lu, 2012). Liver Dis. Pharm. The results showed that carvacrol reduced cell proliferation and provoked apoptosis mediated by mitochondrial membrane potential depolarization, increased mitochondrial calcium and activation of Bax and Cytochrome C expression. Evaluación del uso de los aceites esenciales como alternativa al uso de antibióticos promotores de crecimiento en pollos de engorde. C.C. doi:10.5772/50277, Song, H.-Y., Deng, X.-H., Yuan, G.-Y., Hou, X.-F., Zhu, Z.-D., Zhou, L., et al. species (Martino et al., 2018). Al-mustansiriyah J. Sci. Molecules 26 (2), 410. doi:10.3390/molecules26020410, Carqueijeiro, I., Langley, C., Grzech, D., Koudounas, K., Papon, N., O’Connor, S. E., et al. In addition, a considerable decrease in the expression of cyclin A, B1 and CDK2, as well as an increase in the expression of p21 were observed after treatment with thymol, suggesting that its antitumor effect occurs by inhibiting the PI3K/Akt signaling pathway, via MAPKs, and generation of ROS (Li et al., 2017). HHS Vulnerability Disclosure, Help Targets 19 (1), 113–127. The antineoplastic agents available on the market have different mechanisms of action that impair cell proliferation and/or cause cell death, thereby increasing patient survival rate (Powell et al., 2014; Lee and Park, 2016). Product Description Oil of Oregano has 36 mg carvacrol and thymol from an impressive 60-75% carvacrol oregano oil. Oregano (Origanum vulgare L.) is an aromatic native plant originated from the Mediterranean region and it's traditionally grown in the south of Peru. Sci. [12], Em ratos, o carvacrol é rapidamente metabolizado e excretado. El equipo de HPLC utilizado es Hitachi Elite LaChromm, con el Software EZChrom Elite v3.3.2, equipado con bomba L-2130, válvula gradiente baja presión L-2130ACC, degasificadora L-2000ACC, Interface USB IFU, horno de columnas L-2350, Inyector manual 7725i-006, mezclador cuaternario de solventes; el detector utilizado fue el Hitachi FL Detector L-2485; se utilizó la longitud de onda de excitación: 230 nm, longitud de onda de emisión: 280 nm. Additionally, 26% (n = 18) of in vitro studies reported negative regulation of the anti-apoptotic protein Bcl-2 (Swanton et al., 1999; Kroemer et al., 1998). [4], O carvacrol inibe o crescimento de várias estirpes de bactérias, como Escherichia coli[6] e Bacillus cereus. Cytotoxicity and Morphological Effects Induced by Carvacrol and Thymol on the Human Cell Line Caco-2. doi:10.3923/pjbs.2021.646.655, Allawzi, A., Elajaili, H., Redente, E. F., and Nozik-Grayck, E. (2019). doi:10.1016/j.canlet.2016.03.042, Riaz, S. K., Iqbal, Y., and Malik, M. F. A. Como anticáncer. La determinación de timol y carvacrol en muestras de orégano y otras plantas, ha sido realizado por muchos autores utilizando métodos por HPLC 1-5 y cromatografía de gases con detector FID o de masas. Cisplatin Induces a Mitochondrial-ROS Response that Contributes to Cytotoxicity Depending on Mitochondrial Redox Status and Bioenergetic Functions.

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